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In the current study, following manufacturer’s instruction, 20 μL from each sample, 100 μL of calibrator and 100 μL assay buffer were transferred to separate wells in a Nunc™ 96-well polypropylene MicroWell™ plate (Thermo Scientific Nunc, Roskilde, Denmark)

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[3] Various B vitamins act as cofactors for methylation, including B2 and B12