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In particular, the selection of an appropriate control or comparison group is required to determine, all other things being equal, whether a treatment is efficacious for a given illness, whether it has benefits over other therapies for the illness, and how it can achieve its effects
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To address these limitations, the current protocol employs a Tiron/ferrous ion (Fe 2+ ) reagent, which eliminates the requirement for protein precipitation and effectively resolves the issues associated with earlier methods