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Cells were starved for nitrogen in a carbon free M9 medium for 3 h at 37 °C, harvested by centrifugation and adjusted to the optical density of 3.0 in 20 mM TBS buffer (pH-7.2)
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Size:16.9oz
In order to determine the regulatory mechanism underlying the induction of transsulfuration enzymes, we examined whether CBS and CTH are direct gene targets of the ATF4 transcription factor by performing chromatin immunoprecipitation (ChIP)