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The supernatant was then diluted in the same sonication buffer but without N -lauroylsarcosine, and subjected to immunoprecipitation with ATF4 antibody or IgG control at 4°C overnight
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Results Stapled peptide design and structure–activity relationships To identify inhibitors of the FAT domain of FAK, we employed the hydrocarbon-based stapling approach in the design of peptide analogs with drug-like features such as increased stability, proteolytic resistance, binding affinity, cell permeability, and DMPK properties
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