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A total of 20 µg protein was separated using 10% or 12.5% sodium dodecyl sulphate polyacrylamide gel electrophoresis and blotted onto polyvinylidene fluoride membranes, which were then blocked with 5% non-fat milk diluted in Tris-buffered saline containing 0.05% Tween 20 for 1 h at room temperature
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With these practical considerations in mind, we can turn to the cellular level where some of the most striking evidence lies in Epitalon’s effects on telomeres