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This occurs through altering the concentration of absorbable iron, modulating the intestinal absorption surface area, and affecting the expression of genes related to iron metabolism ( 4.2 Regulation of ferroptosis by gut microbiota metabolites 4.2.1 Short-chain fatty acids Short-chain fatty acids (SCFAs)–mainly acetate, propionate, and butyrate–have been shown to exert multiple beneficial effects in letrozole-induced PCOS rat models, such as improving lipid metabolism and mitigating oxidative stress and inflammation