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Western blot analysis Cells were lysed in lysis buffer [50 mM Tris-HCl (pH 7.5), 1% NP-40, 0.5% (v/v) sodium deoxycholate, 150 mM NaCl and 1× Halt protease and phosphatase inhibitor mixture (Pierce)] on ice for 1 h

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Passwater: Usually GSH supplements are found in the amino acid section of stores, but l-glutathione technically is neither an amino acid nor a protein