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The highest reported oral dose of GABA involved 14 adults (8 gram/kg/day divided into 4 doses) for up to 2 years and was well-tolerated (146)
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cDNA was synthesized from 1 μg of purified RNA using HiScript II Q RT SuperMix (Vazyme) and subjected to quantitative real-time PCR (q-PCR) for analyzing the relative mRNA levels of specific genes with proper primers using ChamQ Universal SYBR qPCR Master Mix (Vazyme)