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Like some of the blood-boosting drugs they purport to mimic, most peptides disappear from the blood quickly, which makes them hard to detect
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For measurement of cytokines, the cDNA was PCR-amplified using the following primers: IFN-β, forward: 5′-CACTGGGTGGAATGAGACTAT-3′,reverse: 5′-GACATCTCCCACGTCAATC-3′, CCL5, forward: 5′-ACTCCCTGCTGCTTTGCCTAC-3′, reverse: 5′-GCGGTTCCTTCGAGTGACA-3′, β-actin, forward: 5′-TAGCACCATGAAGATCAAGAT-3′,reverse: 5′-CCGATCCACACAGAGTACTT-3′